The present invention discloses an array substrate and a manufacturing method thereof, and a display device, the array substrate includes a pixel electrode and a thin film transistor, the pixel electrode includes a first sub-electrode, a first connection part formed integrally with the first sub-electrode, a second sub-electrode, and a second connection part formed integrally with the second sub-electrode, the first sub-electrode and the second sub-electrode are insulated from each other and are disposed in different layers, and both the first connection part and the second connection part are connected to a drain of the thin film transistor. With the present invention, the pixel electrode of the discrete pattern structure can be manufactured to have a narrow gap smaller than the resolution of the exposure machine, to solve the problem that the single-layered pixel electrode of the discrete pattern structure cannot be resolved by the existing exposure machine.