Invention Grant
- Patent Title: Single cell analysis of transposase accessible chromatin
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Application No.: US15842687Application Date: 2017-12-14
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Publication No.: US10400235B2Publication Date: 2019-09-03
- Inventor: Kamila Belhocine , Geoffrey McDermott , Francesca Meschi , Xinying Zheng
- Applicant: 10X Genomics, Inc.
- Applicant Address: US CA Pleasanton
- Assignee: 10X GENOMICS, INC.
- Current Assignee: 10X GENOMICS, INC.
- Current Assignee Address: US CA Pleasanton
- Agency: Wilson, Sonsini, Goodrich & Rosati
- Main IPC: C12N15/10
- IPC: C12N15/10

Abstract:
Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.
Public/Granted literature
- US20180340170A1 SINGLE CELL ANALYSIS OF TRANSPOSASE ACCESSIBLE CHROMATIN Public/Granted day:2018-11-29
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