The invention provides a compliance detecting method of payment applications in a virtualized environment, and pertains to the field of security technology of payment applications. The detecting method is used for compliance detection for PCI DSS isolation demands. The detecting method can determine whether it is a compliance state by analyzing the current virtual machine domain and its connection from data stream, and can also determine whether it is a compliance state by analyzing the purity of network flow of virtual machines. The detecting method is suitable for a virtualized environment and makes the detection of compliance accurate in the virtualized environment, thus being advantageous for guaranteeing the security of payment applications.