A light-emitting substrate and a method for manufacturing the light-emitting substrate are provided. In the light-emitting substrate, each of a plurality of pixel units includes an anode and a light-emitting layer sequentially provided on a base substrate, the light-emitting substrate further includes a cathode layer covering the plurality of pixel units and a region which is between the adjacent ones of the pixel units, an auxiliary electrode is provided below the cathode layer, the auxiliary electrode includes a plurality of auxiliary electrode strips which include a plurality of first auxiliary electrode strips extending along an extension direction of each pixel unit row and/or a plurality of second auxiliary electrode strips extending along an extension direction of each pixel unit column, and at least a part of the plurality of auxiliary electrode strips is electrically connected to the cathode layer.