A microneedle puncture instrument includes a pinch section including a first pinch element and a second pinch element arrayed in an array direction and configured to individually pinch the skin in a pinch direction, which intersects with the array direction, the first pinch element and the second pinch element being connected to each other with a distance therebetween maintained within a predetermined range; and a puncture section configured to puncture the skin while pinching the skin in the pinch direction at a position between the first pinch element and the second pinch element in the array direction, the puncture section including a microneedle for puncturing the skin and a support portion that supports the microneedle.