A display driving method, a display panel and a display device. In the display driving method, the voltage (Vgl) of a gate turning-off signal at least changes once during the period of applying the gate turning-off signal to each gate line (Gate 1, Gate 2, . . . ). A pixel voltage signal is varied as the gate turning-off signal changes. Thus, the variation frequency of the pixel voltage signal within the display time of each frame is increased by changing the gate turning-off signal within the display time of each frame, which is equivalent to increase the refreshing frequency, so that the human eyes cannot recognize flicker.