The present disclosure relates to a pre-5th-Generation (5G) or 5G communication system to be provided for supporting higher data rates Beyond 4th-Generation (4G) communication system such as Long Term Evolution (LTE). The present invention provides a method and a device for cancelling inter-symbol interference in a wireless communication system. A method for a base station in a wireless communication system can comprises the steps of: transmitting multiple synchronous signals through multiple antennas to a terminal; receiving information on a propagation delay difference among the multiple synchronous signals from the terminal; and determining signal transmission timing for each of the multiple antennas on the basis of the information on the propagation delay difference.