A tape cassette stores a first medium having: a first base having a print surface for printing and first adhesive applied on an opposite side of the print surface; and a first separator removably attached to the first base via the first adhesive; and a second medium having: a second base; and a second separator removably attached to the second base. The tape cassette includes a pressing member that is movable in a direction intersecting with a direction of a plane of the print surface, and the pressing member is disposed at a position of, when a pressing force is externally applied to perform bonding of the first medium and the second medium mutually, receiving the pressing force.