An electronic device, an iris recognition method and a non-volatile computer-readable medium are provided. A processor in the electronic device obtains a first and second iris images, and calculates a plurality of first and second feature mark boxes that are non-uniformly arranged according to the first and second iris images. The processor uses the first feature mark boxes to obtain a first and second image features from the first and second iris images respectively, and compares the first and second image features to obtain a first recognition result. The processor uses the second feature mark boxes to obtain a third and fourth image features from the second and first iris images respectively, and compares the third and fourth image features to obtain a second recognition result. The processor determines a similarity degree of the first and second iris images according to the first and second recognition results.