- 专利标题: Addressable flow cell using patterned electrodes
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申请号: US15107882申请日: 2014-12-30
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公开(公告)号: US10537889B2公开(公告)日: 2020-01-21
- 发明人: Chenlu Hou , Byoungsok Jung , Yir-Shyuan Wu , Tarun Khurana
- 申请人: Illumina, Inc.
- 申请人地址: US CA San Diego
- 专利权人: ILLUMINA, INC.
- 当前专利权人: ILLUMINA, INC.
- 当前专利权人地址: US CA San Diego
- 代理机构: Illumina, Inc.
- 国际申请: PCT/US2014/072699 WO 20141230
- 国际公布: WO2015/103225 WO 20150709
- 主分类号: B01L3/00
- IPC分类号: B01L3/00 ; C40B60/00 ; C12Q1/6874 ; C12Q1/68
摘要:
Disclosed are methods and systems concerning flow cells for sequencing a nucleic acid sample that may be characterized by the following components: (a) a substrate having an inner surface facing a library sequencing region, and an outer surface; (b) a plurality of a plurality of forward and reverse amplification primers immobilized over the inner surface and providing a nucleic acid library capture surface of the library sequencing region; (c) a plurality of electrodes disposed along the inner surface directly under at least some of the forward and reverse amplification primers, and configured to provide, when charged, an electric field through the library capture surface and into the library sequencing region; (d) electrical leads connected to the plurality of electrodes to permit the electrodes to be independently addressable; and (e) fluidic couplings configured to deliver a plurality of nucleic acid libraries to the flow cell during different time periods.
公开/授权文献
- US20160318016A1 ADDRESSABLE FLOW CELL USING PATTERNED ELECTRODES 公开/授权日:2016-11-03
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