A laser eye surgery system used to treat vitreous bodies includes a laser source, a ranging subsystem, an integrated optical subsystem, and a patient interface assembly. The laser source produces a treatment beam that includes a plurality of laser pulses. The ranging subsystem produces a source beam used to locate one or more structures of an eye. In some embodiments, the ranging subsystem includes an optical coherence tomography (OCT) pickoff assembly that includes a first optical wedge and a second optical wedge separated from the first optical wedge. The OCT pickoff assembly is configured to divide an OCT source beam into a sample beam and a reference beam. The integrated optical subsystem is used to scan the treatment beam and the sample beam. In other embodiments, Purkinje imaging, Scheimpflug imaging, confocal or nonlinear optical microscopy, ultrasound, stereo imaging, fluorescence imaging, or other medical imaging technique may be used.