A display substrate, a method for preparing the same, and a display device are provided in the embodiments of the disclosure. The display substrate comprises: a flexible substrate, the flexible substrate defining a connection region thereon; an inorganic structure layer on a side of the flexible substrate and in the connection region; a plurality of connection terminals on the inorganic structure layer and in the connection region; and a flexible filling layer in the inorganic structure layer and between two adjacent connection terminals of the plurality of connection terminals.