A display substrate, a display panel, a substrate, a touch substrate and a cutting method of the substrate are disclosed. The display substrate includes a plurality of imagined touch electrodes, a plurality of connection wires, a grounding electrode and a first cutting alignment mark. The plurality of imagined touch electrodes are located at an imagined touch zone of the display substrate; the plurality of connection wires are located at a bonding zone of the display substrate and are connected with the imagined touch electrodes; the grounding electrode is arranged at the bonding zone and is electrically connected with the a plurality of connection wires; the first cutting alignment mark is arranged at a side of the grounding electrode adjacent to the connection wires, and configured that the grounding electrode being able to be cut away from the display substrate by cutting the display substrate through the first cutting alignment mark.