A display panel, a manufacturing method and driving method thereof are provided. The display panel includes a light transmittance adjusting layer and two pixel layers being respectively located on opposite surfaces of light transmittance adjusting layer. Each pixel layer includes multiple light-emitting units arranged in an array. Orthogonal projections of two pixel layers on the light transmittance adjusting layer do not overlap. When light transmittance of light transmittance adjusting layer is higher, light emitted from the light-emitting units on both surfaces of light transmittance adjusting layer is emergent from opposite sides through light transmittance adjusting layer. Two pixel layers display the same picture jointly. When light transmittance of light transmittance adjusting layer is lower, light emitted from light-emitting units on both surfaces of light transmittance adjusting layer cannot be transmitted through light transmittance adjusting layer to reach opposite sides. Two pixel layers display images independently.