A current mirror calibration circuit, coupled to an error amplifier of a pulse-width modulation controller, includes a first voltage generation unit, a second voltage generation unit, a calibration unit and a current mirror circuit. During an initial period, the first voltage generation unit and second voltage generation unit provide a first default voltage and a second default voltage respectively. The current mirror circuit includes a first current unit and a second current unit. The first current unit receives an original current. The second current unit generates a mirror current having a proportional relationship with original current. The first current unit has a first node coupled to the first voltage generation unit and a second node coupled to a third default voltage. The second current unit has a third node coupled to the second voltage generation unit and calibration unit and a fourth node coupled to calibration unit and an output terminal of error amplifier.