Open roof construction for a vehicle comprises a roof opening in a stationary roof part, a panel which by an operating mechanism is movable between a closed position in which it closes the roof opening and an open position in which the panel is moved rearwards to a position at least partially above a part of the stationary roof part behind the roof opening. The operating mechanism comprises a rear moving assembly for guiding and supporting the panel. The open roof construction further comprises seals that are positioned in the roof opening in the region of roof opening edges and which are intended for cooperation with the panel, wherein the rear moving assembly is constructed such that, in the open position of the panel, part of it has come into engagement with the seal that is positioned in the roof opening in the region of the rearward roof opening edge.