A refrigerator appliance and methods of operation are provided herein. The refrigerator appliance may include a cabinet, a storage bin, a lid, an ozone passage, an air conduit, an air handler, and an ozone filter. The storage bin may include a plurality of walls defining a storage volume. The lid may be positioned on the storage bin to selectively cover the storage volume in a closed position. The ozone passage may be defined through at least one of the plurality of walls in fluid communication with the storage volume. The air conduit may be disposed in selective fluid communication with the ozone passage. The air handler may be disposed in fluid communication with the air conduit to direct ozone through the ozone passage. The ozone filter may be disposed in fluid communication with the air conduit to filter ozone passing from the storage volume through the air conduit.