An open roof construction for a vehicle includes a stationary part, a movable first panel and a second panel. The first panel is movably supported by an operating mechanism guided in one or more rails of the stationary part extending at least along a part of the inner roof edge of the opening and rearwardly thereof. The first panel is movable by at least a panel support and is capable of moving with respect to one of the rails and thereby displacing the first panel rearwardly above the second panel. The second panel and/or inner roof edge and/or the stationary part is provided with a deformable seal capable of sealing a gap between the second panel and the adjacent inner roof edge of the fixed roof. The panel support is adapted to deform the seal when it moves through the gap between the second panel and the inner roof edge.