The present disclosure is directed towards systems and methods for validation of a secure socket layer (SSL) certificate of a server for clientless SSL virtual private network (VPN) access. An intermediary device can receive a first request from a client for a clientless SSL VPN connection to a first server. The intermediary device can determine, using a preconfigured policy, that the first server in the first request meets a condition of the preconfigured policy. The intermediary device 801 can perform, responsive to the determination, an action to validate a SSL certificate of the first server using one or more certificate authority (CA) certificate files available to the intermediary device. The one or more CA certificate files can be specified by the preconfigured policy for the action.