The present invention relates to a method of evaluation of molecular binding interactions at a sensing surface, and more particularly to a method for evaluation of screening data obtained from an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface of a biosensor that is independent of interaction models. Preferably the biosensor is a SPR biosensor.The invention also relates to a biosensor system arranged to perform the method and a computer program arranged to control the operation of the biosensor system.