The present disclosure provides a device for collecting semi-volatile or non-volatile substance, including an air nozzle, a front cavity and a collecting body. The air nozzle is configured to eject air to a sample attachment surface. The front cavity has an upper port. The collecting body is sealingly connected to a lower end of the front cavity, inside of which is provided with a cylindrical cavity and a conical cavity arranged vertically coaxially, and bottom of which is provided with a sample outlet. The collecting body is provided with an air intake passage which is non-coplanar with respect to an axis of the cylindrical cavity and is disposed obliquely downward and inward. The collecting body is further provided with an air exhaust passage one end of which is a discharge port connected to the interior of the cylindrical cavity, the other end is connected to an air pump.