A anatomical phantom is disclosed containing a pathological abnormality having fluorescing properties to simulate fluorescence training and/or the simulation of medical procedures, such as fluorescence-based methods for intraoperative visualization and image-guidance. The phantom is made of materials that mimic anatomical biomechanical properties and wherein the pathological abnormality contains fluorescing properties to fluoresce in responsive to UV light excitation. The phantom may be a standalone model or may be used in a complementary simulation kit to provide a more comprehensive training approach.