A touch substrate, a manufacturing method thereof and a display panel are provided. The touch substrate includes: a base, including a touch region and a lead region positioned around the touch region; a plurality of first electrode strips, arranged side by side on the base in the touch region, each of the plurality of first electrode strips including a plurality of first sub-electrodes arranged at intervals; and a plurality of conductive bridges, arranged on the base in the touch region, two adjacent first sub-electrodes in each of the plurality of first electrode strips being electrically connected by one of the plurality of conductive bridges, wherein each of the plurality of conductive bridges includes a main body portion and a branch portion which are directly connected with each other, and at least two branch portions are arranged at least one end of the main body portion.