The present disclosure provides an organic light emitting diode (OLED) and a manufacturing method of the same, and a display device. The OLED comprises a first portion and a second portion which are manufactured by different processes and sequentially stacked, wherein the first portion comprises a first carrier transport layer, a first light emitting layer, and a connecting layer between the first light emitting layer and the second portion, which are sequentially stacked, the second portion comprises a second carrier transport layer, the first light emitting layer comprises an N-type base material and a P-type base material, and the connecting layer comprises a material corresponding to carriers in the first carrier transport layer. The OLED provided by the present disclosure can not only make light emission stable, maintain a good efficiency of light emission, but obtain light with an ideal color and prevent a phenomenon of “unexpected emission of light”.