The disclosure provides a display device, including a substrate, a pixel define layer, a conductive line and a spacer. The pixel define layer is disposed on the substrate, wherein the pixel define layer includes a first opening region and a second opening region, wherein the second opening region is adjacent to the first opening region. The conductive line is disposed on the substrate, wherein in a top view of the display device, the conductive line is located between the first opening region and the second opening region. The spacer is disposed on the substrate, wherein the spacer at least partially overlaps the conductive line.