The present disclosure discloses an electroluminescent substrate plate, a method of manufacturing the same, and a display device. The method includes printing an ink comprising a light-emitting layer material and a solvent capable of dissolving the light-emitting layer material in a display region of a substrate; and printing a solvent in a region other than the display region. Thus, the method can easily obtain an electroluminescent substrate plate, and the manufactured electroluminescent substrate plate can satisfy the dry atmosphere consistency of the display region and the peripheral region during the film formation by inkjet printing, can significantly improve the film thickness uniformity of the display region, and at the same time is beneficial to the design requirements of a large size and a narrow frame.