A circuit and method for detecting pixel potential of a display panel and a display panel is provided. The circuit comprises a multiplexed output selector, at least one detection circuit and at least one signal amplifier. The detection circuit comprises a first TFT receiving a test signal and being connected to the multiplexed output selector. The multiplexed output selector is configured to selectively conduct the first data line, which is connected to a currently-detected sub-pixel unit, to the first TFT in accordance with a reverse clock signal to transmit a pixel potential signal of the currently-detected sub-pixel unit to the first TFT to control the first TFT to transmit the test signal to the signal amplifier. The signal amplifier is configured to receive and amplify the test signal to obtain and output a received signal. The present disclosure is able of measuring real pixel potential of the display panel.