Technologies are generally described for concurrent activation of multiple illumination sources to analyze a sample. A controller may be configured to activate the illumination sources substantially simultaneously, where a current or voltage of each activated illumination source is modulated at a different frequency by respective circuit drivers of the controller. Each activated illumination source may be configured to illuminate the sample with light at a different emission wavelength, and one or more detectors may be configured to detect a composite signal from the sample in response to the illumination. The composite signal may include multiple returned signals, where each returned signal corresponds to light emitted from one of the activated illumination sources at a respective emission wavelength. One or more filters, each associated with a respective modulation frequency of one activated illumination source, may be configured to extract each returned signal from the composite signal for analysis.