A driving method of an organic light-emitting display device, the display device includes a plurality of sub-pixels, each of the sub-pixels includes a driving transistor, an organic light-emitting diode and a sense line connected to the driving transistor and the organic light-emitting diode, the method includes: detecting threshold voltages of driving transistors of the sub-pixels or turn-on voltages of organic light-emitting diodes of the sub-pixels by sense lines; calculating a first driving voltage of a data driving circuit or a second driving voltage applied to anodes of the organic light-emitting diodes according to all detected threshold voltages or detected turn-on voltages respectively; and based on the first driving voltage or the second driving voltage, applying data driving voltages and supply voltages to the sub-pixels.