A display substrate may include a base substrate having a plurality of pixel areas; and a pixel electrode in each of the pixel areas. The pixel electrode may include a vertical stem portion extending in a first direction; a horizontal stem portion extending from the vertical stem portion in a second direction intersecting the first direction; a plurality of main branch portions extending from at least one of the vertical stem portion and the horizontal stem portion in an inclined manner; and a sub-branch portion extending from at least one of the plurality of main branch portions.