A 3D display driving method, a 3D display driving device and a display device are provided. The 3D display driving method includes, during each display period: within a display duration (2n−1)th for a left-eye image of a left-eye image frame, scanning a first gate line in an nth group of gate lines, and within a part of the display duration (2n−1)th for the left-eye image, scanning a second gate line in the nth group of gate lines; and within a display duration (2n)th for a right-eye image of a right-eye image frame, scanning the second gate line in the nth group of gate lines, n being a positive integer smaller than or equal to M, and M being an integer greater than 1.