A speaker adjustment method applicable to an electronic device including two microphones and two speakers is provided. The speaker adjustment method includes the following steps: obtaining two first frequency responses corresponding the two speakers by using one microphone, and obtaining two second frequency responses corresponding the two speakers by using another microphone; calculating a sensitivity difference between the two microphones according to the two first frequency responses and the two second frequency responses, where a distance ratio of distances from the two microphones to one speaker equals to a distance ratio of distances from the two microphones to another speaker; and adjusting outputs of the two speakers according to the sensitivity difference, at least one of the first frequency responses and at least one of the second frequency responses. In addition, an electronic device using the speaker adjustment method is also provided.