Item singulation systems and methods may include an input conveyor, a separation conveyor, a turn conveyor, and an output conveyor that is positioned parallel with the input conveyor. A vision system may determine individual columns of items from among a plurality of items on the input conveyor. Operations of the input conveyor, separation conveyor, turn conveyor, and output conveyor may be controlled substantially as a pull system to transfer individual columns of items to the output conveyor, such that singulated items can be transferred by the output conveyor to downstream processes.