A multi-zone end effector assembly is described in the present disclosure. As a non-limiting example, the multi-zone end effector assembly comprises a manifold that comprises a first interior chamber and a second interior chamber. A first port connects to the first interior chamber, and a second port connects to the second interior chamber. A first compression cup region can be connected to the first interior chamber. The first compression cup region is in air connection with the first port. The first compression cup region is configured to be independently activated in order to create a first vacuum zone. A second compression cup region is connected to the second interior chamber. The second compression region cup is in air connection with the second port. The second compression cup region is configured to be independently activated in order to create a second vacuum zone.