A camera module includes a holder frame, a first lens unit, a second lens unit, a first image-sensing unit and a second image-sensing unit. The holder frame includes a first fixed portion and a second fixed portion. The first lens unit is disposed through the first fixed portion and has a first light incident end and a first light exit end. The second lens unit is disposed through the second fixed portion and has a second light incident end and a second light exit end. The first image-sensing unit is disposed opposite to the first light exit end of the first lens unit. The second image-sensing unit is disposed opposite to the second light exit end of the second lens unit. In addition, a manufacturing method of a camera module is also disclosed.