The present disclosure provides a display panel, a method for preparing the same, and a display panel motherboard. The method for preparing the display panel includes: providing a support substrate; forming a base substrate on a surface of the support substrate; providing a mask plate on another surface; before forming a structure sensitive to laser irradiation on the base substrate, irradiating a surface of the support substrate with a laser, such that the at least one region irradiated by the laser is separated from the support substrate, and a region of the base substrate not irradiated by the laser remains bound to the support substrate, then forming a pixel structure in each of the display panel sub-region of the substrate, to obtain a display panel motherboard; and then cutting the display panel motherboard to obtain at least one separate display panel.