A pixel defining layer and a method for manufacturing the same, a display panel and a method for manufacturing the same, and a display device are provided. The pixel defining layer includes a plurality of pixel defining patterns. Each of the pixel defining patterns includes a first sub-defining pattern and a second sub-defining pattern, the second sub-defining pattern being embedded within the first sub-defining pattern, and an outer edge of the second sub-defining pattern is connected to an inner edge of the first sub-defining pattern, wherein a thickness of the first sub-defining pattern is greater than a thickness of the second sub-defining pattern, and a region surrounded by the second sub-defining pattern is a light emitting region of a light emitting layer.