An array substrate and a manufacturing method thereof, and a display panel are disclosed. The array substrate includes a base substrate, comprising a plurality of sub-pixel regions and inter-sub-pixel regions between adjacent sub-pixel regions; and a first organic functional layer on the base substrate. At least a portion of the first organic functional layer is in the plurality of sub-pixel regions; and the first organic functional layer includes at least one fracture opening, the at least one fracture opening is configured to block a transportation of the carriers between adjacent sub-pixel regions.