The present disclosure provides an insulation detection circuit and method, and a battery management system. The circuit includes a first isolation module, a voltage division module, a signal generation module, first and second sampling points and a processor. A first end of the first isolation module is connected to a power battery, and a second end of the first isolation module is connected to the second sampling point. The signal generation module is connected to the first sampling point and configured to inject an AC signal into the power battery and provide the first sampling point with a first sampled signal. A first end of the voltage division module is connected to the first sampling point, and a second end of the voltage division module is connected to the second sampling point. The processor is configured to calculate an insulation resistance of the power battery.