A cylinder head of an internal combustion engine includes a cylinder head main body which is formed with a main body side plug insertion hole into which an ignition plug is inserted, a cam holder which is fixed to the cylinder head main body and forms cam bearing portions together with the cylinder head main body, wherein the cam bearing portions rotatably support an intake camshaft and an exhaust camshaft, respectively, and wherein the cam holder is formed with a holder side plug insertion hole which communicates with the main body side plug insertion hole and into which the ignition plug is inserted, and a knock pin which is disposed across the main body side plug insertion hole and the holder side plug insertion hole to restrict assembly positions of the cam holder and the cylinder head main body.