Methods, systems, and media for analyzing spherical video content are provided. More particularly, methods, systems, and media for detecting two-dimensional videos placed on a sphere in abusive spherical video content are provided. In some embodiments, the method comprises: receiving an identifier of a spherical video content item, wherein the spherical video content item has a plurality of views; selecting a first frame of the spherical video content item; projecting the first frame of the spherical video content item to a two-dimensional region using a projection defined by a mapping according to which neighboring points of the first frame are mapped to respective neighboring points of the region, and one or more contiguous portions of the frame are each mapped to a corresponding plurality of contiguous portions of the region; identifying an area within the region which meets a criterion indicative of the region having a likelihood above a threshold of including a particular type of content; in response to identifying the area within the region which meets the criterion, analyzing the identified area of the region using a video fingerprinting technique; and, in response to determining that content associated with the identified area of the region matches a reference content item of a plurality of reference content items using the video fingerprinting technique, generating an indication of the match in association with the identifier of the spherical video content item.