Embodiments of the present disclosure provide a method and apparatus for pixel drive control, a display panel and a storage medium. The method includes generating a drive control signal based on display data of target columns of sub-pixels and corresponding target display brightnesses. The drive control signal includes sub-drive control signals having at least two duty cycles. The method includes controlling a pixel drive circuit to output a corresponding pixel drive signal based on the drive control signal. The pixel drive signal includes sub-drive signals having at least two duty cycles corresponding to respective sub-drive control signals. Embodiments of the present disclosure enables different rows of sub-pixels to be driven by signals having different duty cycles, thereby achieving adjustment of charging time for different rows of sub-pixels, to reduce charging difference for rows of sub-pixels and enable their display brightnesses to become uniform.