The present disclosure provides a display substrate, including a base substrate, and a black matrix pattern and a color filter pattern on the base substrate. The black matrix pattern defines one or more display regions of the display substrate and a non-display region surrounding the one or more display regions. The color filter pattern includes a plurality of color filter sub-patterns, each color filter sub-pattern includes a plurality of color filter units, and each color filter unit includes a body portion. At least one of the color filter units further includes an anchoring portion extending from the body portion, and in a direction perpendicular to an extension direction of the body portion, the anchoring portion has a size greater than the body portion. The present disclosure further provides a method for manufacturing the display substrate, a display device and a mask.