A pixel circuit configured to drive a light-emitting element and a driving method therefor, and a display substrate, the pixel circuit comprising: a first switch sub-circuit configured to input, under the control of a first control signal line, a data signal of a data signal line to a first node; a second switch sub-circuit configured to input, under the control of a second control signal line, a first signal of a first signal line to a second node; a driving sub-circuit configured to drive, under the control of the potential of the first node, the light-emitting element to emit light; and a memory sub-circuit configured to store a threshold voltage of the driving sub-circuit before the second switch sub-circuit is turned on in each work cycle of the pixel circuit.