Methods, systems, and computer-readable media for interaction monitoring for virtualized graphics processing are disclosed. Execution of an application is initiated on a virtual compute instance that is implemented using CPU and memory resources of a server. Instruction calls are produced by the execution of the application and sent from the server to a graphics server over a network. The graphics server comprises a physical GPU, and a virtual GPU is implemented using the physical GPU and attached to the virtual compute instance. GPU output is generated at the graphics server based at least in part on execution of the instruction calls using the virtual GPU. A log of interactions between the application and the virtual GPU is stored. The interactions comprise the instruction calls sent to the graphics server and responses to the instruction calls sent to the virtual compute instance.