The present disclosure relates to a method and device for transmitting a signal in a display device. The display device comprises a timing controller and a source driver. The method is applied to any of a plurality of transmitting units of the timing controller. The plurality of transmitting units correspond to a plurality of receiving units of the source driver in a one-to-one relationship. The method comprises: obtaining a scrambled signal by scrambling, via a scrambler of the transmitting unit, a non-identification signal in a signal to be transmitted, the scrambled signal comprising an identification signal and a scrambled non-identification signal; and transmitting the scrambled signal to a corresponding receiving unit. A signal obtained by scrambling a signal X via the scrambler is X16+X5+X4+X3+1, X24+X4+X3+X+1 or X32+X7+X5+X3+X2+X+1. The present disclosure reduces distortion of an image displayed on a display panel.