An array substrate, a display panel and a driving method thereof, and a display device are provided. The array substrate includes a plurality of pixel units arranged in an array, each of the plurality of pixel units includes at least four sub-pixel units corresponding to different colors. When the array substrate displays a frame of image, in each row of pixel units, polarities of pixel voltages received by two sub-pixel units corresponding to the same color in adjacent two pixel units are different; a polarity of each pixel voltage represents the magnitude of each pixel voltage relative to a common voltage.