An anti-agglomeration device for a nanofluid includes a support module, a motion module, a photoacoustic conversion module and a control module. The support module includes a frame and screws and is configured to support the photoacoustic conversion module and the motion module. The photoacoustic conversion module includes a nanosecond laser, a first clamp, a lens, a silica optical fiber, a second clamp, gold nanoparticles and a container and is configured to realize photoacoustic conversion to generate ultrasonic waves. The motion module includes a servo motor, a dovetailed rail, a guide screw, a fixed plate, a slider and a deep groove ball bearing. The motion module is configured to support the photoacoustic conversion module and realize the combined motions of the photoacoustic conversion module. The control module includes a support plate and a CCD camera and is configured to control the motion module in real time.