An I/O request processing method in a virtual machine, a device and a computer readable medium are provided. When a backend device of a Guest kernel begins to process an I/O request chain submitted by a frontend device of the Guest kernel into a shared I/O processing memory, the method obtains a starting position of the I/O request chain in the I/O processing memory; according to the starting position of the I/O request chain, sets an element corresponding to the starting position of the I/O request chain in an I/O state array as a value different from initial values of all positions; and upon finishing processing the I/O request chain, sets an element corresponding to the starting position of the I/O request chain in an I/O request state array as a value identical with initial values.