Methods, systems, and media for presenting an advertisement are provided. In some embodiments, the method comprises: causing an advertisement to be presented in connection with presentation of a video having content associated with an application, wherein the advertisement includes information indicating one or more virtual items associated with the application available for purchase; receiving a user input indicating a purchase of at least one of the one or more virtual items; causing payment information to be collected based on a user account associated with a request for presentation of the video to complete the purchase; and transmitting information indicating the completed purchase to an entity associated with the application.